Hash tag NGS12を拾いました.
Gabe Rudy @gabeinformatics

@GholsonLyon I'm in on "I'll believe it when I see it" popular response to nanopore sequencing. Lots of challenges Where is the data? #NGS12

2012-08-15 23:08:53
Gholson Lyon @GholsonLyon

My opinion: Genia talk seems to state the reailty of nanopore seq now. I have not seen Oxford NP talk. R they really that far ahead? #NGS12

2012-08-15 23:06:17
Gabe Rudy @gabeinformatics

SR Genia's tech: Single mol aspects of biological nanopores (Oxford Nanopores) and put that on a IC detection platform (Ion Torrent) #NGS12

2012-08-15 23:06:13
Bio-IT World @bioitworld

Roever: Genia = what you get if you cross @iontorrent and @oxfordnanopore! Claims >99% single-base detection efficiency. #NGS12

2012-08-15 23:05:09
AllSeq @AllSeq

SR: Genia is claiming >98% accuracy at single base resolution primarily due to improved electronics #NGS12

2012-08-15 23:04:20
Dale Yuzuki @DaleYuzuki

Roever: Other 4th gen SMS - Genia, Oxford, NABsys, IBM/Roche. #NGS12

2012-08-15 23:04:14
Dale Yuzuki @DaleYuzuki

Roever: Compared to ~80% accuracy using same pore molecule. #NGS12

2012-08-15 23:03:30
Bio-IT World @bioitworld

Roever: @oxfordnanopore uses informatics and re-engineering the pore. Genia developing alt chemistries, to be announced shortly. #NGS12

2012-08-15 23:03:19
Dale Yuzuki @DaleYuzuki

Roever: Range of voltages, measure conductance. 98% accurate, single-base, alpha hemolysin pore. #NGS12

2012-08-15 23:03:14
Gabe Rudy @gabeinformatics

SR Genia: Nanopores often have 3-base detection resolution. Need to deconvolve signal. Genia to announce different approach to this. #NGS12

2012-08-15 23:02:36
Dale Yuzuki @DaleYuzuki

Roever: Four different molecules, showing single base differences between the four. 19 experiments, 2700 captures. #NGS12

2012-08-15 23:02:32
Dale Yuzuki @DaleYuzuki

Roever: 30fA noise performance - detection of a few thousand electrons. 3.5 x 10^-12 A, x 1 second time-scale #NGS12

2012-08-15 23:01:48
Bio-IT World @bioitworld

Roever: Most semiconductor chips not made to withstand salt water. Lot of work at Genia to operationalize the surface prep. #NGS12

2012-08-15 23:00:53
Dale Yuzuki @DaleYuzuki

Roever: Review of alpha hemolysin nanopore, looking at more than 1 base due to dimension of the read-head, engineering the protein #NGS12

2012-08-15 23:00:38
Gholson Lyon @GholsonLyon

SR- showing now Alpha-Hemolysis as one protein pore, building on work going back at least a decade. #NGS12

2012-08-15 22:58:58
Gabe Rudy @gabeinformatics

SR Genia: Shooting for ~$1000 price instrument, ~$100 each run for new disposable CMOS chip+pores that does the sequencing. #NGS12

2012-08-15 22:58:10
AllSeq @AllSeq

SR:@geniachip reiterates that their system will cost <$1000 with disposable chips for <$100 #NGS12

2012-08-15 22:58:03
Dale Yuzuki @DaleYuzuki

Roever: No fluidics, no optics, but making the chip is not trivial as they are not designed for salt solutions. #NGS12

2012-08-15 22:57:32
Gholson Lyon @GholsonLyon

SR: showing early prototypes, one called "pipe-b**b" -- looks to be early prototypes indeed. But improving. #NGS12

2012-08-15 22:56:09
Dale Yuzuki @DaleYuzuki

Roever: Picture of prototypes - 'pipe bomb' with amplifier buried in ice. Signal proc done with other equipment. #NGS12

2012-08-15 22:55:42
Dale Yuzuki @DaleYuzuki

Roever: Electronic assembly of bilayer, control of insertion of nanopore, capacitance measurement for sequencing. #NGS12

2012-08-15 22:54:43
AllSeq @AllSeq

SR: Genia's current test system has ~260 nanopores; next system will have >100k @geniachip #NGS12

2012-08-15 22:54:31
Gholson Lyon @GholsonLyon

My opinion: seems very early days on nanopore seq, so I'll be surprised if Oxford NP USB comes out this year. #waiting #hype #NGS12

2012-08-15 22:54:04
Mary Ann Brown @mab_chi

#ngs12 selected poster on #RNA-Seq assembly evaluation for non-model organisms by Shaadi Pooyaei, American Museum of Natural History

2012-08-15 22:53:28
AllSeq @AllSeq

Genia has automated the setup of the lipid bilayer and insertion of the nanopore #NGS12

2012-08-15 22:53:20