NGS12

Dale Yuzuki @DaleYuzuki

Drndic: 1 base 0.1s - 'slow'. Much faster, need to improve the electronics. Rosenstein Nature Methods 2012 (up later). #NGS12

Gholson Lyon @GholsonLyon

MD - cool cartoon video of single molecule going through pore, although "can think of ideas much faster than doing experiments" #NGS12

Bio-IT World @bioitworld

MD: Shows DNA translocation through graphene nanopores, but no sequencing results thus far... Speed apprx 70 bases/microsecond. #NGS12

Dale Yuzuki @DaleYuzuki

Drndic: Simulates ssDNA with 3ns and 1.5nm pore size. Serpentine and springy (my words!) #NGS12

Gholson Lyon @GholsonLyon

MD- very early stage of this, but can detect doubled-over DNA going into pore, as blocks out more ions. #NGS12

Dale Yuzuki @DaleYuzuki

Drndic: Lowers noise via TiO2 deposition. Shows 400bp and 3kb DNA events. #NGS12

AllSeq @AllSeq

RT @bioitworld: MD: Nanopores can be created by knocking out single atoms in graphene lattice. Some would consider a defect, not us. #NGS12

Gholson Lyon @GholsonLyon

MD- Some very cool images of making stuff with graphene. Can heat up the pores. Showing images of pores. #NGS12

Dale Yuzuki @DaleYuzuki

Drndic: e-beam can anneal pores. Multi-layered: Si layer, SiO2, SiN, graphene at top. #NGS12

Bio-IT World @bioitworld

MD: Nanopores can be created by knocking out single atoms in graphene lattice. Some would consider a defect, not us. #NGS12

Dale Yuzuki @DaleYuzuki

Drndic: Graphene control illus., single atom knocked out. Other work on graphene, bridge, chain, other shapes, other electronic apps. #NGS12

Gabe Rudy @gabeinformatics

MD: Physics community can make "beautiful" pores out of graphene. Single atom resolution #NGS12

Gholson Lyon @GholsonLyon

MD- Hole in graphene on top of silicon nitrite layer. And TEM characterization of single layer graphene. #NGS12

Dale Yuzuki @DaleYuzuki

Drndic: Wafer has graphene sheet on it, diced. Can control # of layers of the sheets. Lines, holes. SiNx hole = 20nm, Graphene = 3nm. #NGS12

Gabe Rudy @gabeinformatics

Marija Drndic: Graphene based nanopore sequencing: Potential for 1 base in 100ns. Human genomes could be sequenced // in 10 minutes. #NGS12

Gholson Lyon @GholsonLyon

MD- Counting the # of graphene layers, which they can control. Nanosculpting nanostructures w electron beams. Drndic #NGS12

Dale Yuzuki @DaleYuzuki

Drndic: Video of graphene growth - a deposition layer on a polymer layer, otherwise invisible. #NGS12

Gholson Lyon @GholsonLyon

MD- moving now to graphene nanopores, showing cool video on growing graphene w/ polymer backing. Drndic #NGS12

AllSeq @AllSeq

MD: At native speeds, entire human genome would travel through graphene pore in ~10min #NGS12

Dale Yuzuki @DaleYuzuki

Drndic: One base is 0.3nm - so atomically-thin substrate can detect with better resolution. pore = 3-10nm diameter. #NGS12

Dale Yuzuki @DaleYuzuki

Drndic: 15min. genome: 1 base in 100ns, 10^9 bases = 1000 sec. #NGS12

AllSeq @AllSeq

MD: Using graphene nanopores to count miRNAs (whole molecules, not sequencing bases) #NGS12

Dale Yuzuki @DaleYuzuki

Drndic: Able to discriminate between hmC, C and mC, occupies larger volume and create larger amplitude signals. #NGS12

Gholson Lyon @GholsonLyon

MD-- refers to her work published in Nature Nanotechnology and JACS, discrim. RNA and DNA frags. Drndic #NGS12

Dale Yuzuki @DaleYuzuki

Drndic: Working with NEB - counting microRNA, miRNA122a from rat liver, refer to Nature Nanotech. 2010. #NGS12