NGS12

Rosenstein: Capture molecules, and slow enough to measure. Noise, signal, time, all problems. Faster = more noise. #NGS12

2012-08-15 22:23:53

Rosenstein: Protein vs. solid-state illustrated. Same idea - watching ion flux across the two sides of the pore. Transient signal. #NGS12

2012-08-15 22:23:07

JR: Optical detection of single molecule sequencing: low-noise, weak signal. vs. electrochemical: high-noise, strong signal #NGS12

2012-08-15 22:23:05

JR: 1pA = 6.2 million electrons per second. But the background noise high, so Signal to Noise ratio a prob. #NGS12

2012-08-15 22:22:14

Rosenstein: Patch clamping - 1pA = 6M electrons per sec. Quantized unit is much smaller. SNR challenge is noise. #NGS12

2012-08-15 22:21:41

Rosenstein: SMS optical - consider the Nyquist bandwith #NGS12

2012-08-15 22:20:19

Rosenstein: Fluors: 2500 photons/sec QD's: 500k photons/sec. Brightness = limiting factor. #NGS12

2012-08-15 22:19:51

Rosenstein: SMS = discrete, ensemble is an aggregate number. Thus SMS continuous quantities = discrete. Static quants = dynamic. #NGS12

2012-08-15 22:19:04

Jacob Rosenstein Columbia: Photons are convinient because they are clean. But very inefficient when trying to measure things fast. #NGS12

2012-08-15 22:19:01

Mary Ann Brown @mab_chi

We welcome Michelle Busby to the podium presenting on measuring differential gene expression from RNA-seq data #ngs12

2012-08-15 22:17:49

Rosenstein: "Photons are convenient b/c they are clean." But inefficient due to signal. Transducers, PMTs etc. illustrated. #NGS12

2012-08-15 22:17:33

JR: Ensemble vs. Single-molecule, with optical vs. electronic in both (hopefully). IonTorrent is electronic ensemble. #NGS12

2012-08-15 22:15:56

Rosenstein: Optical - 1 base/hr. Ion Torrent - 1 base/min. SM Optical - 1 base / sec. Electronic SMS - in development. #NGS12

2012-08-15 22:15:17

Rosenstein: Bases per second x parallel. Ensemble vs. SM - optical = thousands x 1 base / sec. #NGS12

2012-08-15 22:14:29

J. Rosenstein: 'Noise and Bandwidth Optimization for Nanopore Sensing at Submicrosecond Temporal Resolution'. #NGS12

2012-08-15 22:12:42

Now up, Jacob Rosenstein, Noise and Bandwidth Optimization for Nanopore sensing at sub-ms temp resolution. #NGS12

2012-08-15 22:12:24

MD: Need 1.1nm sized pore to detect bases by their size alone (SiN pores are currently ~5nm) #NGS12

2012-08-15 22:10:12

Drndic: A&A- Solid-state pores can't get down to 1.5nm (yet) to discriminate sizes. #NGS12

2012-08-15 22:09:35

Drndic: Q&A- non-canonical bases, dsDNA or ssDNA, different sizes = different signals, protein pores it works, not solid state yet. #NGS12

2012-08-15 22:09:01

Drndic: Q&A- everything is done in high-salt, research for the electronics industry doesn't apply there. 'Things go crazy.' #NGS12

2012-08-15 22:07:32

MD- submicrosecond temporal resolution -- need much more work on this. Very early days w/ graphene pores. #NGS12

2012-08-15 22:04:49