HMP2010-09-01
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- いいね！0
McDonnell Genome Institute
@GenomeInstitute
Fodor: CF (cystic fibrosis) airway infection are polymicrobial and complex; look at how responds to antibiotics #HMP2010
2010-09-02 04:32:31
McDonnell Genome Institute
@GenomeInstitute
Fodor: analysis of 23 CF patients who have been on many antibiotics since birth #HMP2010
2010-09-02 04:34:04
McDonnell Genome Institute
@GenomeInstitute
Fodor: antibiotics offering only transient relief to CF patients #HMP2010
2010-09-02 04:35:09
McDonnell Genome Institute
@GenomeInstitute
Fodor: used T_RFLP to fingerprint microbial community in CF patients; old technique but very visual #HMP2010
2010-09-02 04:37:21
McDonnell Genome Institute
@GenomeInstitute
Fodor: CF driven by communities of pathogens that are different across patients #HMP2010
2010-09-02 04:42:35
McDonnell Genome Institute
@GenomeInstitute
Fodor: antibiotics have subtle, transient effect on CF patients; low diversity of microbes associated with severity of symptoms #HMP2010
2010-09-02 04:47:04
Jonathan Eisen
@phylogenomics
Anthony Fodor: how do infections in CF patients respond to antibiotics? Followed CF patients during exacerbation events #HMP2010
2010-09-02 04:33:01
Jonathan Eisen
@phylogenomics
Fodor pronounces T-RFLP "Tee R Flip" while I and I think others usually say "Tee Riff Lip" - I think I like his more #HMP2010
2010-09-02 04:37:59
Jonathan Eisen
@phylogenomics
Fodor - OTU diversity (i.e., number of bacterial species) is lower in severe CF infections #HMP2010 though he notes small sample size
2010-09-02 04:45:23
McDonnell Genome Institute
@GenomeInstitute
Kun Zhang from UCSD on Manipulation and Genome Sequencing of Single Microbial Cells #HMP2010
2010-09-02 05:05:50
McDonnell Genome Institute
@GenomeInstitute
Kun Zhang: Sanger reads provide highly uneven genome coverage. #HMP2010
2010-09-02 05:07:44
McDonnell Genome Institute
@GenomeInstitute
Kun Zhang: microfluidic flow cytometer directs and tracks flow of biological cells; is the size of a penny #HMP2010
2010-09-02 05:09:38
McDonnell Genome Institute
@GenomeInstitute
Kun Zhang: microfluidic flow cytometer initially worked for beads and mammalian cells, but not bacteria. #HMP2010
2010-09-02 05:11:47
McDonnell Genome Institute
@GenomeInstitute
Kun Zhang: Used TSA-FISH labeling of bacteria to improve performance by an order of magnitude. #HMP2010
2010-09-02 05:12:29
McDonnell Genome Institute
@GenomeInstitute
Kun Zhang: Added PMT and signal processing components to improve performance by another order of magnitude. #HMP2010
2010-09-02 05:13:08
Dr. Ruchira S. Datta is also on Mastodon
@emergentnexus
Microblogging Rob Knight's talk http://ff.im/q4aFA #HMP2010
2010-09-02 05:12:24
Dr. Ruchira S. Datta is also on Mastodon
@emergentnexus
Rob Knight has mouse superimposed over Vitruvian Man. :-) #HMP2010
2010-09-02 05:13:44
Dr. Ruchira S. Datta is also on Mastodon
@emergentnexus
Rob Knight: "any time you see a pie, it's a lie" #HMP2010
2010-09-02 05:18:49
Dr. Ruchira S. Datta is also on Mastodon
@emergentnexus
Rob Knight: if your body is a democracy, your gut microbes outvote you! #HMP2010
2010-09-02 05:30:41
Dr. Ruchira S. Datta is also on Mastodon
@emergentnexus
Rob Knight: Cell phones ubiquitous in Malawi due to Moore's Law -- why not DNA sequencing? #HMP2010
2010-09-02 05:32:19
Dr. Ruchira S. Datta is also on Mastodon
@emergentnexus
Rob Knight: http://openmicrobiome.org + http://bioweathermap.org + http://qiime.sourceforge.net #HMP2010
2010-09-02 05:34:58
McDonnell Genome Institute
@GenomeInstitute
Weiwen Zhang from Arizona State on RT-qPCR based Quantitative Analysis of Gene Expression in Single Bacterial Cells #HMP2010
2010-09-02 05:14:54
McDonnell Genome Institute
@GenomeInstitute
W Zhang: measure gene expression in a single bacterial cell requires genetically engineered strains, special instruments, costs $ #HMP2010
2010-09-02 05:17:01
McDonnell Genome Institute
@GenomeInstitute
W Zhang: RT-PCR is more straightforward and scalable in single bacterial cells. #HMP2010
2010-09-02 05:17:42
McDonnell Genome Institute
@GenomeInstitute
W Zhang: From one cell, how many genes can be measured? In one example, for a eukayrotic cell, measured 12 gene responses #HMP2010
2010-09-02 05:29:50