AGBT tweets 2012 (2)
Tom Gingeras on lessons learned from ENCODE and modENCODE #AGBT
2012-02-17 23:40:02TG: over 51,000 genic regions 40.5% protein coding, over 161,000 transcripts. #AGBT
2012-02-17 23:40:36TG: genes don't have a single control region- they have multiple. #AGBT
2012-02-17 23:41:56Gingeras, Lessons learned from complex genomes. 8 transcripts per gene locus more than half are non-coding. #AGBT
2012-02-17 23:42:39RM: fEffects of sequence variation on differential allelic transcription factor occupancy & gene expression. http://t.co/qdAf8OxO #AGBT
2012-02-17 23:42:56#AGBT Tom Gingeras on the transcriptomes: the rule, genes have multiple control points. The exception is 1 gene 1 transcript
2012-02-17 23:42:59#AGBT Tom Gingeras: it is critical to think that most genes have multiple transcription initiation sites
2012-02-17 23:43:14presentation from Oxford @nanopore at the Advances in Genome Biology and Technology (#AGBT) conf coming up at 11.40am (EST) / 4.40pm (GMT).
2012-02-17 23:43:34Gingeras: Number of genes increased 41% since 2004, number of transcripts increased 52% since 2008 #AGBT
2012-02-17 23:43:35Lots of GRCh37 patches for one! RT @GenomeRef: poster 59 if you're at #AGBT to see what GRC is up to with the reference assembly
2012-02-17 23:43:47#agbt TG: thats a fun term i learnt 'irreproducibility detection rate IDR'
2012-02-17 23:46:00#agbt, sorry the term is from TG, but comment is mine :)
2012-02-17 23:46:38Signing on from the Advances in Genome Biology and Technology Conference in Marco Island, FL. Day 2 of talks. #AGBT
2012-02-17 23:47:13Gingeras: cellular addresses RNA for localization: cytoplasmic, nuclear (nucleoli, nucleoplasm, chromatin) #AGBT
2012-02-17 23:47:28Would like to hear more about it: RT @iontorrent Rick Myers, Hudson Alpha, shows 114 CpGs associated with response to antibody therapy #AGBT
2012-02-17 23:47:43#AGBT Tom Gingeras: how do you know low read cnt is background or real transcription? irreproducibility detection rate (IDR) (Bickel et al)
2012-02-17 23:48:00Tom Gingeras: 80% of the genome covered by primary transcripts (90.7% of exons, 79.3% of introns, 35.5% of intergenic seqs) #AGBT.
2012-02-17 23:49:15#AGBT Tom Gingeras: found up to 15K NOVEL multi exon transcripts in intergenic regions, 8K antisense
2012-02-17 23:49:49NB! RT @ElementoLab: #AGBT Tom Gingeras: it is critical to think that most genes have multiple transcription initiation sites #pm101
2012-02-17 23:50:27Gingeras: A very large portion of the genome is appearing in the RNA space as unspliced transcript, very often polyA- and antisense. #AGBT
2012-02-17 23:50:35#AGBT Tom Gingeras: (that was based on RNA-seq in 15 cell lines)
2012-02-17 23:51:15Hear Rich Roberts, NEB CSO, speak at 11:20 in the Plenary Session at #AGBT. http://t.co/4LcUZpwW
2012-02-17 23:51:16#AGBT Tom Gingeras IDR analysis (Li et al 2011). Statistics for two replicates, I know a lot of biologists who will say “two is enough”!
2012-02-17 23:51:49#AGBT Tom Gingeras: intergenic regions migth be much smaller than currently thought, genes might massively overlap
2012-02-17 23:52:47@westr #AGBT cool stuff! He is going into publication now. Stay tuned.
2012-02-17 23:52:56