AGBT tweets 2012 (3)
-
- いいね！0
BF Francis Ouellette
@bffo
JG Kinetic analysis of RNA important, on patient tissues, yes, I strongly agree! #agbt
2012-02-18 23:38:43
Olivier Elemento
@ElementoLab
#AGBT Michel Georges: genetic bubble that came back inserted near Kit, did not contain Kit but piece of chr6-chr29
2012-02-18 23:38:47
David Schlesinger
@djschlesinger
Gray: existing methods for studying RNA kinetics alter transcription, RNA-Seq is better method #AGBT
2012-02-18 23:39:27
David Schlesinger
@djschlesinger
Gray: RNA-Seq densities across introns have negative slopes, why? #AGBT
2012-02-18 23:40:30
David Schlesinger
@djschlesinger
Gray: negative slope is not simply due to RNA polymerase falling off #AGBT
2012-02-18 23:41:15
David Schlesinger
@djschlesinger
Gray: negative slope is due to the intermediate steps in RNA synthesis, starting from first exon and proceeding to further exons #AGBT
2012-02-18 23:42:44
David Schlesinger
@djschlesinger
Gray: negative slope is proportional to transcription initiation rate #AGBT
2012-02-18 23:43:14
BF Francis Ouellette
@bffo
JG introns have negative slope 1st principles: transcription, splicing & degraded steady state #agbt cool to go back to 1st principles!
2012-02-18 23:43:40
Orli Bahcall
@obahcall
Jesse Gray on RNA-seq for in vivo mRNA dynamics from unperturbed cells at steady state #AGBT
2012-02-18 23:44:44
David Schlesinger
@djschlesinger
Gray: some transcripts are longer than diameter of the nucleus, how then do splice sites find each other? #AGBT
2012-02-18 23:45:16
David Schlesinger
@djschlesinger
Gray: splicing of long introns requires waiting for transcription of intron to finish #AGBT
2012-02-18 23:46:00
BF Francis Ouellette
@bffo
JG Large introns are processed as efficiently as short ones #agbt
2012-02-18 23:47:17
David Schlesinger
@djschlesinger
Gray: actually, he disproved that. Splicing does not wait for transcription of next intron. #AGBT
2012-02-18 23:47:47
Olivier Elemento
@ElementoLab
#AGBT Jesse Gray: a lot of mRNA dynamics information can be extracted from RNA-seq read densities across gene bodies and especially introns
2012-02-18 23:49:31
David Schlesinger
@djschlesinger
Gray: describing relationship between RNA-Seq read densities and mRNA processing time #AGBT - too much calculus this early in the morning
2012-02-18 23:50:00
Olivier Elemento
@ElementoLab
#AGBT Jesse Gray: for example splicing of an intron does not have to wait from next intron to be transcribed
2012-02-18 23:50:05
Massgenomics
@Massgenomics
Jesse Gray on RNA-Seq kinetics in vivo: splicing occurs during transcription. Intron size no effect on rate #AGBT
2012-02-18 23:52:22
Olivier Elemento
@ElementoLab
#AGBT Jesse Gray: but splicing of long introns waits for transcription of the intron to finish, no splicing of partially transcribed introns
2012-02-18 23:52:41
David Schlesinger
@djschlesinger
Gray: Lariat formation = 2 minute, exon ligation = 1 minute, intron degradation = 30 seconds #AGBT
2012-02-18 23:54:08
Olivier Elemento
@ElementoLab
#AGBT Jesse Gray: overall intron processing times: lariat formation takes 2mins, exon ligation 1min, intron degradation 0.5min
2012-02-18 23:55:46
David Schlesinger
@djschlesinger
Gray: gene expression by RNA-Seq is bimodal #AGBT
2012-02-18 23:56:08
Olivier Elemento
@ElementoLab
#AGBT Jesse Gray: gene expression by RNA-seq is typically bimodal, low mode is 1-2 copies per cell
2012-02-18 23:57:09
James Hadfield
@coregenomics
Knock knock, can we come in? There are 1000 attendees at #AGBT that want a new sequencer. http://t.co/Un5TZO3n
2012-02-18 23:58:36
拡大
Olivier Elemento
@ElementoLab
#AGBT Jesse Gray: transcription initiation rates can be estimated from 3' read densities of introns, distribution bimodal too
2012-02-19 00:00:19